ELISA, Western Blot
Aliquot and store at -20 grades C. Minimize freezing and thawing.
FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae,
Flotillin 1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Flotillin 1 antibody was made against a 13 amino acid synthetic peptide near the C-Terminus of Flotillin 1.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Flotillin 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 48 kDa band observed in lysates of cell line H460 (calculated MW of 47.4 kDa according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approximately 48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1 ug/mL.