WB, ELISA (peptide)
Polyclonal (goat origin)
Goat (Capra aegagrus hircus)
Flotillin 1 (Biotin Conjugate)
Additional name(s) for this target protein: FLOT1
Western blot: 1-3ug/ml,ELISA (peptide) LOD: 1:2000
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Amino acids SISQVNHKPLRTA were used as the immunogen for this Flotillin 1 antibody.
Optimal dilution of the Flotillin 1 antibody should be determined by the researcher.
This Flotillin 1 antibodyis to be used only for research purposes and not for diagnostics..
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Aliquot and store the Flotillin 1 antibody at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.