Flotillin 1 Antibody

Catalog no.

R31142

Size

0.1mg

Price

389 EUR

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Click here to buy Flotillin 1 Antibody at gentaur.com


Tested applications

WB

Uniprot #

O75955

Category

Antibody

French translation

anticorps

Recognised antigen

Flotillin 1

Conjugation

Unconjugated

Purity

Antigen affinity

Clone

Polyclonal antibody

Recommended dilutions

Western blot: 0.5-1ug/ml

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Intented use

This Flotillin 1 antibodyis to be used only for research purposes and not for diagnostics..

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

An amino acid sequence from the middle region of human FLOT1 (KKAAYDIEVNTRRAQA) was used as the immunogen for this Flotillin 1 antibody.

Notes

The stated application concentrations are suggested starting amounts. Titration of the Flotillin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Storage

After reconstitution, the Flotillin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Description

Flotillin 1 is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 6. Bickel et al.(1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al.(1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al.(2005) found that prostate tumor overexpressed gene-1 interacted with Flotillin1 in detergent-insoluble membrane fractions. Flotillin1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.