ELISA, Western Blot
Aliquot and store at -20 grades C. Minimize freezing and thawing.
FLOT2, flotillin 2, reggie-1, ESA, ESA1, M17S1, Flotillin 2 (epidermal surface antigen 1)
Flotillin 2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Flotillin 2 antibody was made against a 14 amino acid synthetic peptide near the C-Terminus of Flotillin 2.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Flotillin 2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 48 kDa band observed in human cerebellum, lung and testis lysates (calculated MW of 47.1 kDa according to NP_004466.2). Recommended concentration: 0.1-0.3 ug/mL.